The result of the research identified that the fingerprint compound profile of curcuminoid on the four rhizomes was obtained by TLC method using silica gel 60 GF 254 as the stationary phase, chloroform:dichloromethane:methanol (13:6:1) as the mobile phase, and observation under UV 254 nm light and citroborate reagent. This research aims to determine fingerprint compound profile of curcuminoid using Thin Layer Chromatography (TLC) and digital image analysis. Hence, it is necessary to characterize the fingerprints compound profile by a simple and rapid method. Therefore, that they are potentially adulterated and difficult to differentiate in the form of powder. heyneana, and Zingiber cassumunar contain high curcuminoid and have relatively the similar yellow color.
